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Complex characterization of subgingival plaque - use of modern sequencing methods in diagnostics and monitoring of treatment of periodontal diseases
Těšínská, Barbora ; Najmanová, Lucie (advisor) ; Konopásek, Ivo (referee)
Periodontitis is a multifactorial inflammatory disease which can result in a complete loss of teeth. Its main cause is the accumulation of bacteria from the dental plaque followed by massive reaction of the host immune system. It was proved that the composition of oral microbiome (OM) differs in periodontally healthy individuals and patients with periodontitis. This work aims to solve specific parts of the long-term project concerning the taxonomic composition of the OM of periodontally healthy individuals and patients with chronic and aggressive form of the disease. The OM was characterized based on 16s rDNA sequencing. It is evident from the results that the shift in the OM composition occurs prior the development of clincal signs of the disease and that the precise dental care can significantly postpone or even avoid the onset of the disease. When comparing the OM composition in individuals with chronic and aggressive periodontitis, no remarkable differences were detected to explain the faster progress of the aggressive form of the disease. This work also aimed to compare the results obtained by 454 pyrosequencing and Illumina. Both sequencing methods were found to provide statistically comparable results. Illumina MiSeq thus can be employed to build on the former results of the long-term study...
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Creating of an educational program of dental hygiene at DVD for pupils in the first grade of secondary school
KONFRŠT, Miroslav
The aim of the thesis was to create an educational software with the use of DVD player. This DVD is supposed to influence the level of secondary school pupils' awareness about dental hygiene in a positive way. Furthermore, it should increase the students' motivation in taking regular care of their oral cavities. This should be helpful to persist not only their healthy denture but also their overall health. The theoretical part of this thesis is focused on the anatomy of the oral cavity, particularly on the tooth construction and types of teeth. Next, the issue of dental caries and their resemblance in other more serious dental diseases, such as gingivitis or paradontitis, is presented. The attention is paid to both mechanical and chemical tools for cleaning teeth which are the cornerstones of taking proper care about the oral cavity. Second part of the thesis is practical. It is based on evaluation two tests about children's knowledge of the oral cavity and dental hygiene in general. First test was given to the children before presenting the educational software to them and the second one afterwards. Thanks to this approach it was possible to evaluate the progress the children made and subsequently the effectivity of the educational software.
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